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Cl inical Flow Wiki This site is devoted to building a connected community around Clinical Flow Cytometry. It is intended to serve as a resource for information, case examples, e-Learning and resources. We have chosen the wiki format to allow all users to contribute their experience and knowledge to build a vibrant community. We would like to acknowledge Teri Oldaker for her hard work in making analysis templates that are ideal for teaching flow cytometry and Dr. Mark Shenkin for providing case data. To see clinical cases follow: Cases Introduction to Flow Cytometric Analysis FCS Express Reader
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How can aberrant B lymphoblasts in B lymphoblastic leukemia (B-ALL) be distinguished from hematogones?

Hematogones are immature B-cell precursors that are found primarily in the bone marrow from which they arise. They are found in greatest abundance in infants and young children and decline with age. They are frequently increased in regenerating bone marrow after chemotherapy or bone marrow transplant, but also can be increased in other inflammatory, hematologic, oncologic, and inflammatory disorders. They are rarely detected in peripheral blood. It is particularly important to be able to differentiate hematogones from neoplastic B-cell precursors in the diagnosis and monitoring of B-ALL. B-lineage lymphoblasts and hematogones often share immunophenotypic characteristics. Thus, hematogone hyperplasia can be mistaken for B-ALL at diagnosis and regenerating hematogones can be mistaken for minimal residual disease after therapy. Figure 1A shows normal B-lineage maturation. All hematogones are positive for CD19 (not shown) and show variable expression of CD58. The earliest hematogones...
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Atlas Of Hematology by Nivaldo Medeiros, MD

This Atlas has the goal of supplying hematologists, laboratory technicians, medical school students, biologists as well as professionals working in the field of morphology of blood cells. The majority of the smears come from blood and bone marrow samples, however note that also are shown here cavitary fluid materials, scraped, etc. The panchromic stain used is always "Leishman", and are added in addition rare cytochemistry reactions. The pictures taken were scaled up of magnifications such:  x200 ; x400 ;  x630 ; and specially  x1,000  with photomicroscopes  Zeiss  and  Nikon . These pictures show normal cells from blood and bone marrow and continuing to a substantial variety of hematologic diseases, being some of these rather  Fotografias de Maduracion en Medula Osea, alteraciones eritrocitarias, Anemias, Leucemias, etc. Para acceder haga  Click Aqui.
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