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E-Learning Library By Clinical Cytometry Education Network (CCEN)

Internet-based technology is capable of addressing many of the challenges faced in providing global flow cytometry education. According, we strive to expand the availabilty of web-based educational materials and to build a comprehensive electronic library including:

  • Online Courses
    A collection of formal presentations from invited experts covering a broad range of clinical cytometry topics.  Slide-based presentations will be supplemented with notes, audio, video, or real-time interaction (webinar). 

  • Short Instructional Videos
    Short, 5 – 15 minute, videos addressing practical aspects of clinical flow cytometry, including identification of individual cells, an approach to the diagnosis of specific disease entities, how to avoid pitfalls, and technical tips.  

  • Clinical Case Studies
    A comprehensive portfolio of clinical case studies with provision of relevant clinical information, the results of other laboratory testing, and raw cytometry data through the Data Repository. 

  • ICCS Open Educational Resources
    ICCS is working to produce a series of new virtual training tools to facilitate remote flow cytometry education.

Contribute to our online e-learning library by submitting interesting e-course presentations, instructional videos or case studies.
Please send inquires to info@cytoed.org .

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How can aberrant B lymphoblasts in B lymphoblastic leukemia (B-ALL) be distinguished from hematogones?

Hematogones are immature B-cell precursors that are found primarily in the bone marrow from which they arise. They are found in greatest abundance in infants and young children and decline with age. They are frequently increased in regenerating bone marrow after chemotherapy or bone marrow transplant, but also can be increased in other inflammatory, hematologic, oncologic, and inflammatory disorders. They are rarely detected in peripheral blood. It is particularly important to be able to differentiate hematogones from neoplastic B-cell precursors in the diagnosis and monitoring of B-ALL. B-lineage lymphoblasts and hematogones often share immunophenotypic characteristics. Thus, hematogone hyperplasia can be mistaken for B-ALL at diagnosis and regenerating hematogones can be mistaken for minimal residual disease after therapy. Figure 1A shows normal B-lineage maturation. All hematogones are positive for CD19 (not shown) and show variable expression of CD58. The earliest hematogones...